Effect of Solvent Composition on Solubility and Activity of Bovine Leukemia Virus Proteinase

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Title:Effect of Solvent Composition on Solubility and Activity of Bovine Leukemia Virus Proteinase
Creators:
Hrušková-Heidingsfeldová, Olga
Piskáček, Martin
Cubínková, Romana
Zábranský, Aleš
Pichová, Iva
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 61, 8, pp. 1254-1260
Uncontrolled Keywords:Solvent composition, Solubility, BLV proteinase, Specific activity

Abstract

The solubility and activity of bovine leukemia virus proteinase (BLV PR) has been examined as a function of solvent composition in order to find a buffer system which would enable to prepare samples concentrated sufficiently for crystallization attempts and other studies. Low solubility and frequent precipitation was one of the major difficulties in the course of experiments carried out with BLV PR in several laboratories. We found that buffers composed of polyvalent ions, and containing glycerol, ethylene glycol or dimethyl sulfoxide, enabled to concentrate the enzyme up to 2 mg/ml. Presence of 4-20% of DMSO, isopropyl alcohol or ethylene glycol results in activation of BLV PR, while glycerol does not significantly affect the BLV PR activity. Zinc or magnesium ions substituting for sodium ions in commonly used buffers caused decrease both in solubility and activity of BLV PR.

Title:Effect of Solvent Composition on Solubility and Activity of Bovine Leukemia Virus Proteinase
Creators:
Hrušková-Heidingsfeldová, Olga
Piskáček, Martin
Cubínková, Romana
Zábranský, Aleš
Pichová, Iva
Uncontrolled Keywords:Solvent composition, Solubility, BLV proteinase, Specific activity
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:61
Number:8
Page Range:pp. 1254-1260
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19961254UNSPECIFIED
ID Code:1031
Item Type:Article
Deposited On:06 Feb 2009 17:05
Last Modified:06 Feb 2009 16:06

Citation

Hrušková-Heidingsfeldová, Olga; Piskáček, Martin; Cubínková, Romana; Zábranský, Aleš; Pichová, Iva (1996) Effect of Solvent Composition on Solubility and Activity of Bovine Leukemia Virus Proteinase. Collection of Czechoslovak Chemical Communications, 61 (8). pp. 1254-1260. ISSN 0010-0765

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