Human Orosomucoid: Dissociation of Tyrosines in the Systems Water-Low Aliphatic Alcohol

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Title:Human Orosomucoid: Dissociation of Tyrosines in the Systems Water-Low Aliphatic Alcohol
Creators:
Karpenko, Vladimír
Horálková, Jana
Kodíček, Milan
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 62, 10, pp. 1533-1543
Uncontrolled Keywords:Tyrosine dissociation, Orosomucoid, Alcohols effects, Isopermittivity conditions, UV-spectroscopy

Abstract

The influence of methanol, ethanol, propan-1-ol, and propan-2-ol on the dissociation of tyrosines of human serum orosomucoid (acid α<sub>1</sub>-glycoprotein) was studied. The content of alcohols was chosen so that their solutions had the same value of the relative permittivity. Then, the contribution of electrostatic effects was equal in all cases and the observed differences between aqueous and alcoholic solutions reflected other, nonelectrostatic effects in the studied systems. Analysis of the spectrophotometric titration curve revealed three kinds of tyrosine groups (<i>n</i><sub>1</sub>, <i>n</i><sub>2</sub>, <i>n</i><sub>3</sub>), but only the values p<i>K</i><sub>1</sub> and p<i>K</i><sub>2</sub> could be calculated. The observed differences of p<i>K</i> values in aqueous and mixed media are discussed and compared with the structural changes of the orosomucoid molecule.

Title:Human Orosomucoid: Dissociation of Tyrosines in the Systems Water-Low Aliphatic Alcohol
Creators:
Karpenko, Vladimír
Horálková, Jana
Kodíček, Milan
Uncontrolled Keywords:Tyrosine dissociation, Orosomucoid, Alcohols effects, Isopermittivity conditions, UV-spectroscopy
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:62
Number:10
Page Range:pp. 1533-1543
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19971533UNSPECIFIED
ID Code:1229
Item Type:Article
Deposited On:06 Feb 2009 17:07
Last Modified:06 Feb 2009 16:07

Citation

Karpenko, Vladimír; Horálková, Jana; Kodíček, Milan (1997) Human Orosomucoid: Dissociation of Tyrosines in the Systems Water-Low Aliphatic Alcohol. Collection of Czechoslovak Chemical Communications, 62 (10). pp. 1533-1543. ISSN 0010-0765

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