Spectrophotometric Determination of Bile Acids; An Evaluation

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Title:Spectrophotometric Determination of Bile Acids; An Evaluation
Creators:
Fini, Adamo
Zuman, Petr
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 58, 1, pp. 53-61

Abstract

Bile acids can be determinated by measuring absorbance in the ranges between 290 and 310 nm or between 370 and 390 nm of species which are formed when bile acids react in 77.8 wt.% sulfuric acid at 70 °C for 30 min. The absorption bands at about 300 nm correspond to a formation of allylic carbocations, those at about 380 nm to dienylic carbocations. The absorbance was found to be linear function of concentration between 1 and 5 . 10<sup>-5</sup> mol l<sup>-1</sup>. Measurements of absorbance were compared for 11 bile acids. The method can be used for analysis of dilute solutions of these acids, e.g. in estimating their solubility.

Title:Spectrophotometric Determination of Bile Acids; An Evaluation
Creators:
Fini, Adamo
Zuman, Petr
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:58
Number:1
Page Range:pp. 53-61
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19930053UNSPECIFIED
ID Code:126
Item Type:Article
Deposited On:06 Feb 2009 16:58
Last Modified:06 Feb 2009 15:58

Citation

Fini, Adamo; Zuman, Petr (1993) Spectrophotometric Determination of Bile Acids; An Evaluation. Collection of Czechoslovak Chemical Communications, 58 (1). pp. 53-61. ISSN 0010-0765

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