Characterization of Glycoprotein Fraction from Carp Pituitaries Isolated Using Concanavalin A as the Affinity Ligand

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Title:Characterization of Glycoprotein Fraction from Carp Pituitaries Isolated Using Concanavalin A as the Affinity Ligand
Creators:
Hulová, Irena
Barthová, Jana
Ryšlavá, Helena
Kašička, Václav
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 63, 3, pp. 434-440
Uncontrolled Keywords:Gonadotropins, Capillary electrophoresis, Glycoproteins, Carp pituitary, Hormones

Abstract

Glycoproteins that have affinity to Concanavalin A were isolated from the acetone-dried pituitaries of common carp (<i>Cyprinus carpio</i> L.). Two fractions of glycoproteins were separated using gel chromatography on Superdex 75HR. The fraction with lower molecular weight (30 000) corresponding to the carp gonadotropin cGtH II was composed of two subunits as determined using SDS-PAGE. This protein fraction was further divided into four components using reversed-phase HPLC. Two fractions were pure α and β subunits of cGtH II as follows from immunodetection and from determination of <i>N</i>-terminal amino acid sequences. The other two were a mixture of α and β subunits as was also revealed by <i>N</i>-terminal analysis. Capillary electrophoresis was also used for characterization of isolated glycoproteins.

Title:Characterization of Glycoprotein Fraction from Carp Pituitaries Isolated Using Concanavalin A as the Affinity Ligand
Creators:
Hulová, Irena
Barthová, Jana
Ryšlavá, Helena
Kašička, Václav
Uncontrolled Keywords:Gonadotropins, Capillary electrophoresis, Glycoproteins, Carp pituitary, Hormones
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:63
Number:3
Page Range:pp. 434-440
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19980434UNSPECIFIED
ID Code:1311
Item Type:Article
Deposited On:06 Feb 2009 17:08
Last Modified:06 Feb 2009 16:08

Citation

Hulová, Irena; Barthová, Jana; Ryšlavá, Helena; Kašička, Václav (1998) Characterization of Glycoprotein Fraction from Carp Pituitaries Isolated Using Concanavalin A as the Affinity Ligand. Collection of Czechoslovak Chemical Communications, 63 (3). pp. 434-440. ISSN 0010-0765

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