Isoenzymes of GMP Kinase from L1210 Cells: Isolation and Characterization

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Title:Isoenzymes of GMP Kinase from L1210 Cells: Isolation and Characterization
Creators:
Krejčová, Romana
Horská, Květoslava
Votruba, Ivan
Holý, Antonín
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 64, 3, pp. 559-570
Uncontrolled Keywords:L1210 cells, Phosphorylation, Nucleotide kinase, Phosphates, Enzymes, isolation, GMP kinase, Isoenzymes, Nucleotides

Abstract

Guanylate kinase from a mouse leukemic L1210 cells has been purified nearly to homogeneity for the first time. It consists of five isoenzymes with p<i>I</i> 5.95, 5.50, 5.08, 4.83 and 4.51, respectively. The native enzyme is a monomer with a relative molecular mass 25 000. The kinetic constants <i>K</i><sub>m</sub>, <i>V</i><sub>max</sub> and <i>k</i><sub>cat</sub>/<i>K</i><sub>m</sub> of isoenzymes were estimated. The purified GMP kinase is absolutely specific to GMP and/or dGMP as phosphate acceptor but has a broad specificity to nucleoside 5'-triphosphates as phosphate donors. <p>

Title:Isoenzymes of GMP Kinase from L1210 Cells: Isolation and Characterization
Creators:
Krejčová, Romana
Horská, Květoslava
Votruba, Ivan
Holý, Antonín
Uncontrolled Keywords:L1210 cells, Phosphorylation, Nucleotide kinase, Phosphates, Enzymes, isolation, GMP kinase, Isoenzymes, Nucleotides
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:64
Number:3
Page Range:pp. 559-570
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19990559UNSPECIFIED
ID Code:1499
Item Type:Article
Deposited On:06 Feb 2009 17:10
Last Modified:06 Feb 2009 16:10

Citation

Krejčová, Romana; Horská, Květoslava; Votruba, Ivan; Holý, Antonín (1999) Isoenzymes of GMP Kinase from L1210 Cells: Isolation and Characterization. Collection of Czechoslovak Chemical Communications, 64 (3). pp. 559-570. ISSN 0010-0765

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