Conditions Resulting in Formation of Properly Assembled Retroviral Capsids within Inclusion Bodies of Escherichia coli

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Title:Conditions Resulting in Formation of Properly Assembled Retroviral Capsids within Inclusion Bodies of Escherichia coli
Creators:
Rumlová-Kliková, Michaela
Pichová, Iva
Hunter, Eric
Ruml, Tomáš
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 64, 8, pp. 1348-1356
Uncontrolled Keywords:Native proteins, Inclusion body, Bacterial expression, Retroviruses, Retroviral capsid assembly

Abstract

It has been generally accepted that inclusion bodies (IBs) formed in <i>Escherichia coli</i> consist of non-biologically active aggregated proteins, which are stabilized by non-productive interactions. We show here that bacterial expression of a retroviral capsid polyprotein results in formation of insoluble IBs that contain fully assembled viral particles connected with amorphous material. The efficiency of IBs formation and capsid assembly was not significantly affected by changes in induction temperature, pH of cultivation medium or the level of expression. <p>

Title:Conditions Resulting in Formation of Properly Assembled Retroviral Capsids within Inclusion Bodies of Escherichia coli
Creators:
Rumlová-Kliková, Michaela
Pichová, Iva
Hunter, Eric
Ruml, Tomáš
Uncontrolled Keywords:Native proteins, Inclusion body, Bacterial expression, Retroviruses, Retroviral capsid assembly
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:64
Number:8
Page Range:pp. 1348-1356
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19991348UNSPECIFIED
ID Code:1561
Item Type:Article
Deposited On:06 Feb 2009 17:10
Last Modified:06 Feb 2009 16:10

Citation

Rumlová-Kliková, Michaela; Pichová, Iva; Hunter, Eric; Ruml, Tomáš (1999) Conditions Resulting in Formation of Properly Assembled Retroviral Capsids within Inclusion Bodies of Escherichia coli. Collection of Czechoslovak Chemical Communications, 64 (8). pp. 1348-1356. ISSN 0010-0765

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