Linear Heptapeptides Containing DNA-Intercalators. Synthesis and Interaction with DNA

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Title:Linear Heptapeptides Containing DNA-Intercalators. Synthesis and Interaction with DNA
Creators:
Matušina, Zbyněk
Olbřímková, Radomíra
Votavová, Hana
Neumann, Josef
Hradilek, Martin
Souček, Milan
Maloň, Petr
Kodíček, Milan
Stibor, Ivan
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 64, 9, pp. 1419-1432
Uncontrolled Keywords:Quinoxalines, Peptides, Sedimentation analysis, CD spectroscopy, Intercalation, UV spectroscopy, Acridines, DNA, Topoisomerase I

Abstract

Five peptides of general structure X-Ser-Pro-Thr-Ser-Pro-Ser-Y (X = Tyr, quinoxaline- 2-carbonyl, acridine-9-carbonyl, Y = Tyr, (quinoxalin-2-yl)amino) were prepared using standard solid-phase peptide synthesis technique. Their interaction with DNA (calf thymus DNA and plasmids pUC9, p∆NS and pGEMEX) was studied using UV and CD spectroscopy, sedimentation analysis and agarose gel electrophoresis after treatment with topoisomerase I. In contrast to earlier findings (Suzuki M.: <i>Nature</i> <strong>1990</strong>, <i>344</i>, 562) intercalation into DNA structure has not been proved for any compound studied. <p>

Title:Linear Heptapeptides Containing DNA-Intercalators. Synthesis and Interaction with DNA
Creators:
Matušina, Zbyněk
Olbřímková, Radomíra
Votavová, Hana
Neumann, Josef
Hradilek, Martin
Souček, Milan
Maloň, Petr
Kodíček, Milan
Stibor, Ivan
Uncontrolled Keywords:Quinoxalines, Peptides, Sedimentation analysis, CD spectroscopy, Intercalation, UV spectroscopy, Acridines, DNA, Topoisomerase I
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:64
Number:9
Page Range:pp. 1419-1432
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19991419UNSPECIFIED
ID Code:1567
Item Type:Article
Deposited On:06 Feb 2009 17:10
Last Modified:06 Feb 2009 16:10

Citation

Matušina, Zbyněk; Olbřímková, Radomíra; Votavová, Hana; Neumann, Josef; Hradilek, Martin; Souček, Milan; Maloň, Petr; Kodíček, Milan; Stibor, Ivan (1999) Linear Heptapeptides Containing DNA-Intercalators. Synthesis and Interaction with DNA. Collection of Czechoslovak Chemical Communications, 64 (9). pp. 1419-1432. ISSN 0010-0765

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