Chicken Pituitary Glycoproteins: New Isolation Method

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Title:Chicken Pituitary Glycoproteins: New Isolation Method
Creators:
Ryšlavá, Helena
Krešlová, Jana
Barthová, Jana
Barth, Tomislav
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 64, 9, pp. 1510-1516
Uncontrolled Keywords:Gonadotropins, Mass spectrometry, Glycoproteins, Chicken pituitary, Hormones, Proteins, Ion-exchange chromatography, HPLC

Abstract

A new method for isolation of glycoproteins from chicken pituitaries was applied. The procedure consist of chromatography on ConA-Sepharose and by HPLC on S Hyper D and Vydac C4 columns. The hormonal activity of the glycoproteins was tested by determining their stimulatory effect on cAMP or testosterone production. Molecular weights of the products of tryptic cleavage of the hormone were determined using mass spectrometry (MALDI TOF). A comparison of the values obtained with theory shows that the protein is the β-unit of chicken luteinizing hormone. <p>

Title:Chicken Pituitary Glycoproteins: New Isolation Method
Creators:
Ryšlavá, Helena
Krešlová, Jana
Barthová, Jana
Barth, Tomislav
Uncontrolled Keywords:Gonadotropins, Mass spectrometry, Glycoproteins, Chicken pituitary, Hormones, Proteins, Ion-exchange chromatography, HPLC
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:64
Number:9
Page Range:pp. 1510-1516
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19991510UNSPECIFIED
ID Code:1575
Item Type:Article
Deposited On:06 Feb 2009 17:10
Last Modified:06 Feb 2009 16:10

Citation

Ryšlavá, Helena; Krešlová, Jana; Barthová, Jana; Barth, Tomislav (1999) Chicken Pituitary Glycoproteins: New Isolation Method. Collection of Czechoslovak Chemical Communications, 64 (9). pp. 1510-1516. ISSN 0010-0765

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