Colour-Monitored Solid-Phase Multiple Peptide Synthesis under Low-Pressure Continuous-Flow Conditions. Synthesis of Medium-Size Peptides: The Propart of Human Procathepsin D and the Growth-Hormone Releasing Factor

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Title:Colour-Monitored Solid-Phase Multiple Peptide Synthesis under Low-Pressure Continuous-Flow Conditions. Synthesis of Medium-Size Peptides: The Propart of Human Procathepsin D and the Growth-Hormone Releasing Factor
Creators:
Vágner, Josef
Krchňák, Viktor
Pícha, Josef
Píchová, Drahomíra
Fusek, Martin
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 58, 2, pp. 435-444

Abstract

The synthesis of two medium-size peptide amides, the propart of human procathepsin D (1-45) and the bovine growth-hormon releasing factor (1-44) has been accomplished by the solid-phase multiple peptide synthesis under low-pressure continuous-flow conditions on standard methylbenzhydrylamine polystyrene-based resin in concatenated flow reactors with adjustable volume. The completeness of acylation of resin-bound free amino groups was monitored by the acid-base indicator bromophenol blue. The efficiency of the coupling reaction was increased by exposure to elevated temperature in an ultrasonic bath. Crude peptides were purified on a mass-overloaded Vydac C18 reversed-phase semipreparative HPLC column. The synthetic propart of human procathepsin D (1-45) exhibited inhibition activity to bovine cathepsin D and four other aspartic proteases. Significantly increased plasma concentration in gilts was determined after application of synthetic bovine growth-hormone releasing factor (1-44).

Title:Colour-Monitored Solid-Phase Multiple Peptide Synthesis under Low-Pressure Continuous-Flow Conditions. Synthesis of Medium-Size Peptides: The Propart of Human Procathepsin D and the Growth-Hormone Releasing Factor
Creators:
Vágner, Josef
Krchňák, Viktor
Pícha, Josef
Píchová, Drahomíra
Fusek, Martin
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:58
Number:2
Page Range:pp. 435-444
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19930435UNSPECIFIED
ID Code:167
Item Type:Article
Deposited On:06 Feb 2009 16:58
Last Modified:06 Feb 2009 15:58

Citation

Vágner, Josef; Krchňák, Viktor; Pícha, Josef; Píchová, Drahomíra; Fusek, Martin (1993) Colour-Monitored Solid-Phase Multiple Peptide Synthesis under Low-Pressure Continuous-Flow Conditions. Synthesis of Medium-Size Peptides: The Propart of Human Procathepsin D and the Growth-Hormone Releasing Factor. Collection of Czechoslovak Chemical Communications, 58 (2). pp. 435-444. ISSN 0010-0765

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