Comparison of Procedures for Isolation of Monoterpene Hydrocarbons from Fresh Needles of Picea abies and Picea omorica

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Title:Comparison of Procedures for Isolation of Monoterpene Hydrocarbons from Fresh Needles of Picea abies and Picea omorica
Creators:
Holubová, Valerie
Chvílíčková, Iva
Kubáň, Vlastimil
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 65, 7, pp. 1073-1081
Uncontrolled Keywords:Monoterpenes, Isolation, Mass spectrometry, Extractions, Supercritical fluid extraction, Gas chromatography, GC-MS, Natural products, Plants, Steam distillations

Abstract

Extraction procedures (steam distillation, supercritical fluid extraction and solvent extraction) for isolation of monoterpene hydrocarbons from fresh needles of <i>Picea abies</i> and <i>Picea omorica</i> were optimised. The procedures were compared with the aim of minimizing consumption of needles and improving the extraction efficiency and repeatability. An influence of homogenisation procedures and storage conditions (liquid nitrogen, -18 and 4 °C) on the total content and composition of essential oils was studied. Cryogenic grinding (liquid nitrogen) combined with the extraction with cold hexane (extraction time 2 h) and subsequent GC-MS determination in freshly homogenised needles gives the best results (1.5-4 times better extraction efficiency, RSD &lt; 10% for <i>P. abies</i> and &lt; 25% for <i>P. omorica</i>). Limits of detections (3 S/N) for individual monoterpene hydrocarbons from units to tens of ng/g and recoveries 97.2-101.4% were found in fresh needles (calculated to fresh weight). While cooling to 4 °C is unacceptable, freezing at -18 °C for the period of 18 days in the dark gives also good results. <p>

Title:Comparison of Procedures for Isolation of Monoterpene Hydrocarbons from Fresh Needles of Picea abies and Picea omorica
Creators:
Holubová, Valerie
Chvílíčková, Iva
Kubáň, Vlastimil
Uncontrolled Keywords:Monoterpenes, Isolation, Mass spectrometry, Extractions, Supercritical fluid extraction, Gas chromatography, GC-MS, Natural products, Plants, Steam distillations
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:65
Number:7
Page Range:pp. 1073-1081
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc20001073UNSPECIFIED
ID Code:1701
Item Type:Article
Deposited On:06 Feb 2009 17:11
Last Modified:06 Feb 2009 16:11

Citation

Holubová, Valerie; Chvílíčková, Iva; Kubáň, Vlastimil (2000) Comparison of Procedures for Isolation of Monoterpene Hydrocarbons from Fresh Needles of Picea abies and Picea omorica. Collection of Czechoslovak Chemical Communications, 65 (7). pp. 1073-1081. ISSN 0010-0765

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