Purification and Characterisation of a Vitellogenin Derived Aminopeptidase from Rainbow Trout Eggs

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Title:Purification and Characterisation of a Vitellogenin Derived Aminopeptidase from Rainbow Trout Eggs
Creators:
Kronovetr, Jakub
Barthová, Jana
Barth, Tomislav
Ryšlavá, Helena
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 65, 7, pp. 1198-1204
Uncontrolled Keywords:Aminopeptidases, Fish eggs, Enzymes, 4-Nitroanilides, Rainbow trout, Vitellogenin

Abstract

An aminopeptidase specific for N-terminal alanine was isolated from the rainbow trout (<i>Oncorhynchus mykiss</i>) maturing eggs using a simple procedure. The enzyme is composed of two identical units each of MW 16&nbsp;000, separable by disulfide reduction. The enzyme has a high content of acid amino acids and its N-terminal sequence is the following <sup>1</sup>EVNAVKCSMV<sup>10</sup>RDTLTTFNNK<sup>20</sup>KYQIN<sup>25</sup>. The sequence is identical with that of the rainbow trout vitellogenin precursor beginning with the amino acid in position 1&nbsp;385. No peptidase activity has been so far observed in proteins derived from this precursor. The enzyme activity is partially blocked by Na<sub>4</sub>-EDTA but it is not inactivated by 4-(chloromercuri)benzoate, phenylmethanesulfonyl fluoride or pepstatin A. <p>

Title:Purification and Characterisation of a Vitellogenin Derived Aminopeptidase from Rainbow Trout Eggs
Creators:
Kronovetr, Jakub
Barthová, Jana
Barth, Tomislav
Ryšlavá, Helena
Uncontrolled Keywords:Aminopeptidases, Fish eggs, Enzymes, 4-Nitroanilides, Rainbow trout, Vitellogenin
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:65
Number:7
Page Range:pp. 1198-1204
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc20001198UNSPECIFIED
ID Code:1712
Item Type:Article
Deposited On:06 Feb 2009 17:11
Last Modified:06 Feb 2009 16:12

Citation

Kronovetr, Jakub; Barthová, Jana; Barth, Tomislav; Ryšlavá, Helena (2000) Purification and Characterisation of a Vitellogenin Derived Aminopeptidase from Rainbow Trout Eggs. Collection of Czechoslovak Chemical Communications, 65 (7). pp. 1198-1204. ISSN 0010-0765

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