Phosphorylation of Purine (Phosphonomethoxy)alkyl Derivatives by Mitochondrial AMP Kinase (AK2 Type) from L1210 Cells

[img]PDF - Authorized users only
Language: English
223Kb
Title:Phosphorylation of Purine (Phosphonomethoxy)alkyl Derivatives by Mitochondrial AMP Kinase (AK2 Type) from L1210 Cells
Creators:
Krejčová, Romana
Horská, Květoslava
Votruba, Ivan
Holý, Antonín
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 65, 10, pp. 1653-1668
Uncontrolled Keywords:PMPA, AMP kinase, L1210 cells, Acyclic nucleotide analogues, PMEDAP, Phosphates, Phosphonates, Antivirals, AK2, PMEA, Enzymatic phosphorylation, Nucleotides

Abstract

Substrate activity of purine (phosphonomethoxy)alkyl derivatives towards mitochondrial AMP kinase (AK2 type) from L1210 cells was studied. The native AMP kinase, purified nearly to homogeneity, is a monomer with molecular weight 26 kDa. The purified AMP kinase is specific for natural adenine nucleotides (AMP and dAMP) as phosphate acceptors but has a broad specificity to nucleoside 5'-triphosphates as phosphate donors. In addition to adenine acyclic nucleotide analogues, the enzyme is capable of phosphorylating also analogous derivatives containing 2,6-diaminopurine moiety. Kinetic data show that the substrate activity of these acyclic nucleoside phosphonates towards AK2 isoenzyme decreases in the order (<i>S</i>)-HPMPA &gt; (<i>R</i>)-PMPA &gt; PMEA &gt; PMEDAP &gt; (<i>S</i>)-PMPDAP &gt; (<i>R</i>)-PMPDAP &gt;&gt; (<i>S</i>)-PMPA. Acyclic nucleotide analogues do not exhibit any inhibitory activity towards AK2 isoenzyme. <p>

Title:Phosphorylation of Purine (Phosphonomethoxy)alkyl Derivatives by Mitochondrial AMP Kinase (AK2 Type) from L1210 Cells
Creators:
Krejčová, Romana
Horská, Květoslava
Votruba, Ivan
Holý, Antonín
Uncontrolled Keywords:PMPA, AMP kinase, L1210 cells, Acyclic nucleotide analogues, PMEDAP, Phosphates, Phosphonates, Antivirals, AK2, PMEA, Enzymatic phosphorylation, Nucleotides
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:65
Number:10
Page Range:pp. 1653-1668
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc20001653UNSPECIFIED
ID Code:1747
Item Type:Article
Deposited On:06 Feb 2009 17:12
Last Modified:06 Feb 2009 16:12

Citation

Krejčová, Romana; Horská, Květoslava; Votruba, Ivan; Holý, Antonín (2000) Phosphorylation of Purine (Phosphonomethoxy)alkyl Derivatives by Mitochondrial AMP Kinase (AK2 Type) from L1210 Cells. Collection of Czechoslovak Chemical Communications, 65 (10). pp. 1653-1668. ISSN 0010-0765

Repository Staff Only: item control page