Identification of HL60 Proteins Affected by 5-Aminolevulinic Acid-Based Photodynamic Therapy Using Mass Spectrometric Approach

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Title:Identification of HL60 Proteins Affected by 5-Aminolevulinic Acid-Based Photodynamic Therapy Using Mass Spectrometric Approach
Creators:
Halada, Petr
Man, Petr
Grebeňová, Dana
Hrkal, Zbyněk
Havlíček, Vladimír
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 66, 11, pp. 1720-1728
Uncontrolled Keywords:Protein identification, Mass spectrometry, Peptide mass fingerprinting, Electrospray ionisation, Matrix-assisted laser desorption/ionisation, Photodynamic therapy, MALDI

Abstract

A combination of mass spectrometric techniques was used for identification of HL60 leukemia cell proteins affected by 5-aminolevulinic acid-based photodynamic therapy (ALA-PDT). We compared two-dimensional electrophoresis (2-DE) protein maps of ALA-treated non-irradiated and irradiated cells and found extensive changes in the proteome of HL60 cells. The silver-stained 2-DE pattern of HL60 proteins contained more than 1 350 spots. Matrix-assisted laser desorption/ionisation mass spectrometry and microcapillary liquid chromatography/tandem mass spectrometry have identified twelve proteins differing in their intensity or position following ALA-PDT. Several endoplasmic reticulum, mitochondrial, ribosomal and cytoplasmic proteins were determined showing the impact of ALA-PDT-mediated cytotoxicity on some cellular pathways. <p>

Title:Identification of HL60 Proteins Affected by 5-Aminolevulinic Acid-Based Photodynamic Therapy Using Mass Spectrometric Approach
Creators:
Halada, Petr
Man, Petr
Grebeňová, Dana
Hrkal, Zbyněk
Havlíček, Vladimír
Uncontrolled Keywords:Protein identification, Mass spectrometry, Peptide mass fingerprinting, Electrospray ionisation, Matrix-assisted laser desorption/ionisation, Photodynamic therapy, MALDI
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:66
Number:11
Page Range:pp. 1720-1728
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc20011720UNSPECIFIED
ID Code:1889
Item Type:Article
Deposited On:06 Feb 2009 17:13
Last Modified:06 Feb 2009 16:13

Citation

Halada, Petr; Man, Petr; Grebeňová, Dana; Hrkal, Zbyněk; Havlíček, Vladimír (2001) Identification of HL60 Proteins Affected by 5-Aminolevulinic Acid-Based Photodynamic Therapy Using Mass Spectrometric Approach. Collection of Czechoslovak Chemical Communications, 66 (11). pp. 1720-1728. ISSN 0010-0765

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