Analysis of Underivatized Brassinosteroids by HPLC/APCI-MS. Occurrence of 3-Epibrassinolide in Arabidopsis thaliana

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Title:Analysis of Underivatized Brassinosteroids by HPLC/APCI-MS. Occurrence of 3-Epibrassinolide in Arabidopsis thaliana
Creators:
Konstantinova, Olga V.
Antonchick, Andrey P.
Oldham, Neil J.
Zhabinskii, Vladimir N.
Khripach, Vladimir A.
Schneider, Bernd
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 66, 12, pp. 1729-1734
Uncontrolled Keywords:Mass spectrometry, Isotopic labelling, Steroids, Metabolism, <i>Arabidopsis thaliana</i>, Brassinosteroids, HPLC/APCI-MS

Abstract

In the course of investigations on brassinosteroid (BS) biosynthetic and metabolic pathways in plants, an approach using HPLC/APCI-MS analysis of underivatized BS for their identification in plant material has been developed. Its application to root-callus suspension cultures of <i>Arabidopsis thaliana</i> led to the first identification of 3-epibrassinolide as natural brassinosteroid. <p>

Title:Analysis of Underivatized Brassinosteroids by HPLC/APCI-MS. Occurrence of 3-Epibrassinolide in Arabidopsis thaliana
Creators:
Konstantinova, Olga V.
Antonchick, Andrey P.
Oldham, Neil J.
Zhabinskii, Vladimir N.
Khripach, Vladimir A.
Schneider, Bernd
Uncontrolled Keywords:Mass spectrometry, Isotopic labelling, Steroids, Metabolism, <i>Arabidopsis thaliana</i>, Brassinosteroids, HPLC/APCI-MS
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:66
Number:12
Page Range:pp. 1729-1734
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc20011729UNSPECIFIED
ID Code:1890
Item Type:Article
Deposited On:06 Feb 2009 17:13
Last Modified:06 Feb 2009 16:13

Citation

Konstantinova, Olga V.; Antonchick, Andrey P.; Oldham, Neil J.; Zhabinskii, Vladimir N.; Khripach, Vladimir A.; Schneider, Bernd (2001) Analysis of Underivatized Brassinosteroids by HPLC/APCI-MS. Occurrence of 3-Epibrassinolide in Arabidopsis thaliana. Collection of Czechoslovak Chemical Communications, 66 (12). pp. 1729-1734. ISSN 0010-0765

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