Detection and Quantification of 7-Hydroxydehydroepiandrosterone Epimers in Three Body Fluids

[img]PDF - Authorized users only
Language: English
87Kb
Title:Detection and Quantification of 7-Hydroxydehydroepiandrosterone Epimers in Three Body Fluids
Creators:
Hampl, Richard
Hill, Martin
Stárka, Luboslav
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 67, 1, pp. 10-18
Uncontrolled Keywords:7-Hydroxydehydroepiandrosterone, Saliva, Steroids, Hormones, GC-MS, Radioimmunoassay, Blood, Semen, Metabolites

Abstract

3β,7α-Dihydroxyandrost-5-en-17-one (<strong>1</strong>) (7α-OH-DHEA) and its 7β-hydroxy epimer <strong>2</strong> (7β-OH-DHEA) - 7α- and 7β-hydroxydehydroepiandrosterone - were detected and quantified in three human body fluids: in blood serum, saliva and ejaculate. Specific radioimmunoassay and gas chromatography-mass spectrometry have been used. For the first time the data on changes of these dehydroepiandrosterone metabolites are reported for a representative group of healthy subjects of both sexes (172 females and 217 males) during the life span. The serum levels of both 7-hydroxydehydroepiandrosterone epimers in serum and also in semen were in the low nanomolar range, while concentrations by one order of magnitude lower were found in saliva, but still within the detection limit. The results will serve as a basis for comparative studies of 7-hydroxydehydroepiandrosterone levels under various pathophysiological conditions, with a particular respect to autoimmune disorders. <p>

Title:Detection and Quantification of 7-Hydroxydehydroepiandrosterone Epimers in Three Body Fluids
Creators:
Hampl, Richard
Hill, Martin
Stárka, Luboslav
Uncontrolled Keywords:7-Hydroxydehydroepiandrosterone, Saliva, Steroids, Hormones, GC-MS, Radioimmunoassay, Blood, Semen, Metabolites
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:67
Number:1
Page Range:pp. 10-18
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc20020010UNSPECIFIED
ID Code:1901
Item Type:Article
Deposited On:06 Feb 2009 17:13
Last Modified:06 Feb 2009 16:13

Citation

Hampl, Richard; Hill, Martin; Stárka, Luboslav (2002) Detection and Quantification of 7-Hydroxydehydroepiandrosterone Epimers in Three Body Fluids. Collection of Czechoslovak Chemical Communications, 67 (1). pp. 10-18. ISSN 0010-0765

Repository Staff Only: item control page