Amperometric Determination of Glucose with a Carbon Paste Biosensor

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Title:Amperometric Determination of Glucose with a Carbon Paste Biosensor
Creators:
Schachl, Klemens
Turkušić, Emir
Komersová, Alena
Bartoš, Martin
Moderegger, Helmut
Švancara, Ivan
Alemu, Hailemichael
Vytřas, Karel
Jimenez-Castro, Maria
Kalcher, Kurt
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 67, 3, pp. 302-313
Uncontrolled Keywords:Flow-injection analysis, Carbon paste electrodes, Oxidases, Glucose, Amperometry, Biosensors

Abstract

A simple glucose biosensor has been developed by bulk modification of a carbon paste electrode with glucose oxidase as a biocomponent and manganese dioxide as a mediator. The sensor was employed as amperometric detector in a flow-injection system at 21 °C in 0.2 M phosphate buffer (pH 7.5). At an applied working potential of 0.48 V <i>vs</i> Ag/AgCl and a flow rate of 0.2 ml min<sup>-1</sup>, the sensor exhibited well reproducible amperometric response to glucose. A linear relation between the peak current and the analyte concentration was found between 20 and 500 mg l<sup>-1</sup> glucose with a detection limit (3σ) of 10.5 mg l<sup>-1</sup> glucose. The sensor can be operated continuously for 12 h without any loss in the signal height and can be used for the determination of glucose in white wine samples. <p>

Title:Amperometric Determination of Glucose with a Carbon Paste Biosensor
Creators:
Schachl, Klemens
Turkušić, Emir
Komersová, Alena
Bartoš, Martin
Moderegger, Helmut
Švancara, Ivan
Alemu, Hailemichael
Vytřas, Karel
Jimenez-Castro, Maria
Kalcher, Kurt
Uncontrolled Keywords:Flow-injection analysis, Carbon paste electrodes, Oxidases, Glucose, Amperometry, Biosensors
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:67
Number:3
Page Range:pp. 302-313
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc20020302UNSPECIFIED
ID Code:1923
Item Type:Article
Deposited On:06 Feb 2009 17:14
Last Modified:06 Feb 2009 16:14

Citation

Schachl, Klemens; Turkušić, Emir; Komersová, Alena; Bartoš, Martin; Moderegger, Helmut; Švancara, Ivan; Alemu, Hailemichael; Vytřas, Karel; Jimenez-Castro, Maria; Kalcher, Kurt (2002) Amperometric Determination of Glucose with a Carbon Paste Biosensor. Collection of Czechoslovak Chemical Communications, 67 (3). pp. 302-313. ISSN 0010-0765

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