24-Epibrassinolide at Subnanomolar Concentrations Modulates Growth and Production Characteristics of a Mouse Hybridoma

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Title:24-Epibrassinolide at Subnanomolar Concentrations Modulates Growth and Production Characteristics of a Mouse Hybridoma
Creators:
Franěk, František
Eckschlager, Tomáš
Kohout, Ladislav
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 68, 11, pp. 2190-2200
Uncontrolled Keywords:Monoclonal antibodies, Cell cycle, 24-Epibrassinolides, Brassinosteroids, Mitochondrial membrane potential, Hybridoma cells, Plant growth regulators, Steroids

Abstract

Brassinosteroids are known to stimulate plant growth and to possess antistress activities in plants. This work was aimed at exploring possible beneficial effects of 24-epibrassinolide on cultured mammalian cells. A mouse hybridoma was cultured either in standard serum-free medium, or in medium diluted to 30%, in which the cells underwent nutritional stress. Steady-state parameters of semicontinuous cultures conducted at 24-epibrassinolide concentrations from 10<sup>-16</sup> to 10<sup>-9</sup> mol l<sup>-1</sup> were evaluated. Typical effects of the agent found both in standard and in diluted media were (i) increase in the value of mitochondrial membrane potential, (ii), drop of intracellular antibody level, (iii) increase in the fraction of the cells in the G<sub>0</sub>/G<sub>1</sub> phase, and (iv) decrease in the fraction of the cells in the S phase. Alleviation of nutritional stress manifested itself in cultures conducted in diluted media. Viable cell density was significantly higher (relative to control) at 24-epibrassinolide concentrations 10<sup>-13</sup> and 10<sup>-12</sup> mol l<sup>-1</sup>. The results of this exploratory study show that the plant hormone 24-epibrassinolide may induce perturbations in the cell division mechanism, in mitochondria performance, and in secreted protein synthesis in a mammalian cell line. At the lowest brassinosteroid concentrations, the number of steroid molecules in the culture was of the same order of magnitude as the number of viable cells in the culture. This implies involvement of a complex cascade mechanism, through which the steroid molecule induces alterations in gene expression leading finally to significant changes in cell culture parameters. <p>

Title:24-Epibrassinolide at Subnanomolar Concentrations Modulates Growth and Production Characteristics of a Mouse Hybridoma
Creators:
Franěk, František
Eckschlager, Tomáš
Kohout, Ladislav
Uncontrolled Keywords:Monoclonal antibodies, Cell cycle, 24-Epibrassinolides, Brassinosteroids, Mitochondrial membrane potential, Hybridoma cells, Plant growth regulators, Steroids
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:68
Number:11
Page Range:pp. 2190-2200
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc20032190UNSPECIFIED
ID Code:2179
Item Type:Article
Deposited On:06 Feb 2009 17:16
Last Modified:06 Feb 2009 16:16

Citation

Franěk, František; Eckschlager, Tomáš; Kohout, Ladislav (2003) 24-Epibrassinolide at Subnanomolar Concentrations Modulates Growth and Production Characteristics of a Mouse Hybridoma. Collection of Czechoslovak Chemical Communications, 68 (11). pp. 2190-2200. ISSN 0010-0765

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