Monitoring of DNA Adducts in Humans and 32P-Postlabelling Methods. A Review

[img]PDF - Authorized users only
Language: English
198Kb
Title:Monitoring of DNA Adducts in Humans and 32P-Postlabelling Methods. A Review
Creators:
Stiborová, Marie
Rupertová, Martina
Hodek, Petr
Frei, Eva
Schmeiser, Heinz H.
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 69, 3, pp. 476-498
Uncontrolled Keywords:DNA adducts, DNA damage, DNA modifications, Biomonitoring, Carcinogens, <sup>32</sup>P-Postlabelling assay, Risk assessment

Abstract

DNA adduct formation in humans is a promising biomarker for elucidating the molecular epidemiology of cancer. For detection of DNA adducts, the most widely used methods include mass spectroscopy, fluorescence spectroscopy, immunoassays and <sup>32</sup>P-postlabelling. Among them, the <sup>32</sup>P-postlabelling method appears to meet best the criteria of sensitivity and amount of DNA needed, and, therefore, is one of the most appropriate methods for biomonitoring of human DNA adducts. Most classes of carcinogens have been subjected to <sup>32</sup>P-postlabelling analysis, ranging from bulky and/or aromatic compounds to small and/or aliphatic compounds; it has also been used, with modifications, to detect apurinic sites in DNA, oxidative damage to DNA, UV-induced photodimers and, to a lesser extent, DNA damage caused by cytotoxic drugs. It has been used in human biomonitoring studies to detect DNA damage from occupational exposure to carcinogens, and also from environmental (i.e. non-occupational) exposures. It has also led to the discovery of the presence of numerous modifications in DNA arising from endogenous processes. The principle of the method is the enzymatic digestion of DNA to nucleotides, 5'-labelling of these nucleotides with an isotopically labelled phosphate group, and the resolution, detection and quantitation of the labelled products. Since the development of the original procedure in the early 1980s, many methods have been developed to increase the sensitivity by enrichment of modified nucleotides prior to labelling. The review presents the individual <sup>32</sup>P-postlabelling techniques (standard procedure, enrichment methods) and a critical evaluation of these assays, besides reviewing the applications of the method to different DNA modifications, and its utilization in human biomonitoring studies. A review with 179 references. <p>

Title:Monitoring of DNA Adducts in Humans and 32P-Postlabelling Methods. A Review
Creators:
Stiborová, Marie
Rupertová, Martina
Hodek, Petr
Frei, Eva
Schmeiser, Heinz H.
Uncontrolled Keywords:DNA adducts, DNA damage, DNA modifications, Biomonitoring, Carcinogens, <sup>32</sup>P-Postlabelling assay, Risk assessment
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:69
Number:3
Page Range:pp. 476-498
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc20040476UNSPECIFIED
ID Code:2225
Item Type:Article
Deposited On:06 Feb 2009 17:16
Last Modified:06 Feb 2009 16:16

Citation

Stiborová, Marie; Rupertová, Martina; Hodek, Petr; Frei, Eva; Schmeiser, Heinz H. (2004) Monitoring of DNA Adducts in Humans and 32P-Postlabelling Methods. A Review. Collection of Czechoslovak Chemical Communications, 69 (3). pp. 476-498. ISSN 0010-0765

Repository Staff Only: item control page