Aggregated Forms of Bull Seminal Plasma Proteins and Their Heparin-Binding Activity

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Title:Aggregated Forms of Bull Seminal Plasma Proteins and Their Heparin-Binding Activity
Creators:
Jelínková, Petra
Ryšlavá, Helena
Liberda, Jiří
Jonáková, Věra
Tichá, Marie
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 69, 3, pp. 616-630
Uncontrolled Keywords:Aggregated forms of proteins, Heparin-binding proteins, Sperm, Fertilization, Affinity chromatography, Seminal plasma, Bull seminal plasma proteins

Abstract

Heparin-binding activity of bull seminal plasma proteins was shown to be dependent on their aggregation state. The protein fraction interacting with immobilized heparin was characterized by large polydispersity in the region of molecular weight of 60 000-10 000, while that not retained on the affinity carrier was present as aggregates with molecular weight &gt;100 000. Components of heparin-binding and non-heparin-binding fractions were separated by RP HPLC (reversed-phase HPLC) and analyzed by SDS (sodium dodecyl sulfate) electrophoresis and N-terminal sequencing. Size exclusion chromatography of whole seminal plasma and heparin-binding proteins in the presence of D-fructose (as a component of seminal plasma) showed that the region of molecular weights of protein-associated forms was shifted to lower values. An increase of heparin-binding activity of bull proteins, as determined by ELBA (Enzyme-Linked Binding Assay), correlates with a decrease of their aggregation state. The modulation of the aggregation state of bull proteins by seminal plasma components and, in this way, also of their heparin-binding properties suggests possible mechanisms for capacitation mediated by these proteins. <p>

Title:Aggregated Forms of Bull Seminal Plasma Proteins and Their Heparin-Binding Activity
Creators:
Jelínková, Petra
Ryšlavá, Helena
Liberda, Jiří
Jonáková, Věra
Tichá, Marie
Uncontrolled Keywords:Aggregated forms of proteins, Heparin-binding proteins, Sperm, Fertilization, Affinity chromatography, Seminal plasma, Bull seminal plasma proteins
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:69
Number:3
Page Range:pp. 616-630
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc20040616UNSPECIFIED
ID Code:2232
Item Type:Article
Deposited On:06 Feb 2009 17:16
Last Modified:06 Feb 2009 16:16

Citation

Jelínková, Petra; Ryšlavá, Helena; Liberda, Jiří; Jonáková, Věra; Tichá, Marie (2004) Aggregated Forms of Bull Seminal Plasma Proteins and Their Heparin-Binding Activity. Collection of Czechoslovak Chemical Communications, 69 (3). pp. 616-630. ISSN 0010-0765

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