Chicken Egg Yolk as an Excellent Source of Highly Specific Antibodies Against Cytochromes P450

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Title:Chicken Egg Yolk as an Excellent Source of Highly Specific Antibodies Against Cytochromes P450
Creators:
Hodek, Petr
Koblas, Tomáš
Rýdlová, Helena
Kubíčková, Božena
Šulc, Miroslav
Hudeček, Jiří
Stiborová, Marie
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 69, 3, pp. 659-673
Uncontrolled Keywords:Immunoglobulins, Chicken egg, Crayfish, Antigens, IgY application, Antibodies, Cytochrome P450, Yolk antibody, Human liver, Immunoassays

Abstract

Using chicken antibodies IgY (purified from egg yolks) against mammalian cytochromes P450 and by means of cytochrome P450 marker substrates, we found for the first time the presence of hepatopancreatic cytochrome P450 in crayfish <i>Orconectes limosus</i> (an inducible cytochrome P450 2B-like enzyme) and we were able to detect and quantify cytochrome P450 1A1 in microsomes of human livers. Expression levels of cytochrome P450 1A1 in human livers constituted less than 0.6% of the total hepatic cytochrome P450 complement. The results obtained in our study are clear examples that chicken IgY are suitable for cytochrome P450 detection and quantification. Due to the evolutionary distance, chicken IgY reacts with more epitopes on a mammalian antigen, which gives an amplification of the signal. Moreover, this approach offers many advantages over common mammalian antibody production since chicken egg is an abundant source of antibodies (about 100 mg IgY/yolk) and the egg collection is a non-invasive technique. In the case of antibodies against cytochrome P450 2B4, we documented fast and steady production of highly specific immunoglobulins. Thus, chicken antibodies should be considered as a good alternative to and/or superior substitute for conventional polyclonal antibody produced in mammals. <p>

Title:Chicken Egg Yolk as an Excellent Source of Highly Specific Antibodies Against Cytochromes P450
Creators:
Hodek, Petr
Koblas, Tomáš
Rýdlová, Helena
Kubíčková, Božena
Šulc, Miroslav
Hudeček, Jiří
Stiborová, Marie
Uncontrolled Keywords:Immunoglobulins, Chicken egg, Crayfish, Antigens, IgY application, Antibodies, Cytochrome P450, Yolk antibody, Human liver, Immunoassays
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:69
Number:3
Page Range:pp. 659-673
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc20040659UNSPECIFIED
ID Code:2235
Item Type:Article
Deposited On:06 Feb 2009 17:16
Last Modified:06 Feb 2009 16:16

Citation

Hodek, Petr; Koblas, Tomáš; Rýdlová, Helena; Kubíčková, Božena; Šulc, Miroslav; Hudeček, Jiří; Stiborová, Marie (2004) Chicken Egg Yolk as an Excellent Source of Highly Specific Antibodies Against Cytochromes P450. Collection of Czechoslovak Chemical Communications, 69 (3). pp. 659-673. ISSN 0010-0765

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