Mercury Electrodes in Nucleic Acid Electrochemistry: Sensitive Analytical Tools and Probes of DNA Structure. A Review

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Title:Mercury Electrodes in Nucleic Acid Electrochemistry: Sensitive Analytical Tools and Probes of DNA Structure. A Review
Creators:
Fojta, Miroslav
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 69, 4, pp. 715-747
Uncontrolled Keywords:DNA structure, DNA interactions, Mercury film electrodes, DNA hybridization, Mercury electrodes, Electrochemistry, Nucleic acids, Electrochemical sensors, DNA damage, Solid amalgam electrodes

Abstract

This review is devoted to applications of mercury electrodes in the electrochemical analysis of nucleic acids and in studies of DNA structure and interactions. At the mercury electrodes, nucleic acids yield faradaic signals due to redox processes involving adenine, cytosine and guanine residues, and tensammetric signals due to adsorption/desorption of polynucleotide chains at the electrode surface. Some of these signals are highly sensitive to DNA structure, providing information about conformation changes of the DNA double helix, formation of DNA strand breaks as well as covalent or non-covalent DNA interactions with small molecules (including genotoxic agents, drugs, etc.). Measurements at mercury electrodes allow for determination of small quantities of unmodified or electrochemically labeled nucleic acids. DNA-modified mercury electrodes have been used as biodetectors for DNA damaging agents or as detection electrodes in DNA hybridization assays. Mercury film and solid amalgam electrodes possess similar features in the nucleic acid analysis to mercury drop electrodes. On the contrary, intrinsic (label-free) DNA electrochemical responses at other (non-mercury) solid electrodes cannot provide information about small changes of the DNA structure. A review with 188 references. <p>

Title:Mercury Electrodes in Nucleic Acid Electrochemistry: Sensitive Analytical Tools and Probes of DNA Structure. A Review
Creators:
Fojta, Miroslav
Uncontrolled Keywords:DNA structure, DNA interactions, Mercury film electrodes, DNA hybridization, Mercury electrodes, Electrochemistry, Nucleic acids, Electrochemical sensors, DNA damage, Solid amalgam electrodes
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:69
Number:4
Page Range:pp. 715-747
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc20040715UNSPECIFIED
ID Code:2239
Item Type:Article
Deposited On:06 Feb 2009 17:16
Last Modified:06 Feb 2009 16:16

Citation

Fojta, Miroslav (2004) Mercury Electrodes in Nucleic Acid Electrochemistry: Sensitive Analytical Tools and Probes of DNA Structure. A Review. Collection of Czechoslovak Chemical Communications, 69 (4). pp. 715-747. ISSN 0010-0765

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