Electrochemistry of Dopamine β-Hydroxylase in Adsorbed State at a Gold Electrode

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Title:Electrochemistry of Dopamine β-Hydroxylase in Adsorbed State at a Gold Electrode
Creators:
Ion, Ana
Ljones, Torbjørn
Banica, Florinel G.
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 69, 4, pp. 759-775
Uncontrolled Keywords:Glycoproteins, Adsorption, Copper protein

Abstract

Dopamine β-hydroxylase (DBH) adsorbed on gold electrode was investigated by electrochemical and quartz crystal microbalance methods. It was found that DBH can adsorb irreversibly on a gold electrode under open circuit conditions. In a neutral phosphate buffer, the adsorbed layer is stable to desorption within the potential range of 0.6 to -0.7 V vs Ag|AgCl (1&nbsp;M KCl) electrode. At potentials positive than 0.8 V, adsorbed DBH undergoes an electrochemical oxidation involving probably tyrosine, tryptophan and histidine residues. This process is accompanied by DBH desorption and gold oxidation. In the cathodic potential region, from -0.2 to -0.7 V, DBH exhibits two reductions assigned to disulfide groups. No charge transfer reactions involving the copper centres in DBH have been noticed. However, DBH can bind reversibly copper(II) ions, which are not involved in the enzymatic activity and their presence was proved by voltammetry. The compactness of the adsorbed film was checked by cyclic voltammetry of selected redox probes at the DBH-coated electrode. <p>

Title:Electrochemistry of Dopamine β-Hydroxylase in Adsorbed State at a Gold Electrode
Creators:
Ion, Ana
Ljones, Torbjørn
Banica, Florinel G.
Uncontrolled Keywords:Glycoproteins, Adsorption, Copper protein
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:69
Number:4
Page Range:pp. 759-775
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc20040759UNSPECIFIED
ID Code:2241
Item Type:Article
Deposited On:06 Feb 2009 17:16
Last Modified:06 Feb 2009 16:16

Citation

Ion, Ana; Ljones, Torbjørn; Banica, Florinel G. (2004) Electrochemistry of Dopamine β-Hydroxylase in Adsorbed State at a Gold Electrode. Collection of Czechoslovak Chemical Communications, 69 (4). pp. 759-775. ISSN 0010-0765

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