Second and Third Derivatives of UV Spectra as a Tool for Identification of Major Anthocyanins from Aronia melanocarpa Extract, Separated Using Reversed-Phase High-Performance Liquid Chromatography

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Title:Second and Third Derivatives of UV Spectra as a Tool for Identification of Major Anthocyanins from Aronia melanocarpa Extract, Separated Using Reversed-Phase High-Performance Liquid Chromatography
Creators:
Minkiewicz, Piotr
Pliszka, Barbara
Dziuba, Jerzy
Oszmiański, Jan
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 69, 7, pp. 1443-1452
Uncontrolled Keywords:UV derivative spectroscopy, HPLC, Photodiode-array detector, <i>Aronia melanocarpa</i>, Natural products, Glycosides, Anthocyanins

Abstract

The aim of the study was to propose a method for identification of major anthocyanins in black chokeberry: cyanidin 3-<i>O</i>-β-D-galactopyranoside and cyanidin 3-<i>O</i>-α-L-arabinopyranoside, using reversed-phase high-performance liquid chromatography on line with UV spectroscopy. The similarity indexes between standard spectra and spectra of major components of black chokeberry extract were calculated. The calculations were performed for zero-order spectra and first-fifth derivatives. Only the second and third derivatives of UV spectra provided correct identification of the compounds investigated. Third derivatives of spectra gave higher values of the parameters (calculated using Student's test) indicating statistical significance between similarity indexes to the derivatives of spectra of anthocyanin standards. Third derivative UV spectroscopy may be thus recommended as a tool to identify anthocyanins. <p>

Title:Second and Third Derivatives of UV Spectra as a Tool for Identification of Major Anthocyanins from Aronia melanocarpa Extract, Separated Using Reversed-Phase High-Performance Liquid Chromatography
Creators:
Minkiewicz, Piotr
Pliszka, Barbara
Dziuba, Jerzy
Oszmiański, Jan
Uncontrolled Keywords:UV derivative spectroscopy, HPLC, Photodiode-array detector, <i>Aronia melanocarpa</i>, Natural products, Glycosides, Anthocyanins
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:69
Number:7
Page Range:pp. 1443-1452
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc20041443UNSPECIFIED
ID Code:2283
Item Type:Article
Deposited On:06 Feb 2009 17:17
Last Modified:06 Feb 2009 16:17

Citation

Minkiewicz, Piotr; Pliszka, Barbara; Dziuba, Jerzy; Oszmiański, Jan (2004) Second and Third Derivatives of UV Spectra as a Tool for Identification of Major Anthocyanins from Aronia melanocarpa Extract, Separated Using Reversed-Phase High-Performance Liquid Chromatography. Collection of Czechoslovak Chemical Communications, 69 (7). pp. 1443-1452. ISSN 0010-0765

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