Investigations on the Reduction of Molecular Oxygen on Polyphenol Oxidase-Modified Electrode

[img]PDF - Authorized users only
Language: English
96Kb
Title:Investigations on the Reduction of Molecular Oxygen on Polyphenol Oxidase-Modified Electrode
Creators:
Jiang, Hui
Shi, Chun-Hua
Xie, Yong-Shu
Liu, Qing-Liang
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 70, 3, pp. 305-312
Uncontrolled Keywords:Polyphenol oxidase, Modified electrodes, Electrochemistry, Nafion modified electrode, Enzymes, Square-wave voltammetry, Oxygen detection

Abstract

Polyphenol oxidase (PPO, E.C.1.10.3.1) from <i>Nicotiana tobacum</i>, PPO II, shows a different behavior from that of PPOs from other sources. A one-electron oxygen reduction on PPO II - Nafion modified glassy carbon electrode surface has been observed by square-wave voltammetry. The cathodic peak has been found to be proportional to the concentration of dissolved oxygen. The optimum salt concentration was 0.2 mol/l. The study of its dependence on pH (4-9) indicates that the reduction of oxygen is accompanied by a one-proton-transfer process. Temperature shows little influence on the cathodic peak. The electrode storage has also been studied to evaluate its stability. <p>

Title:Investigations on the Reduction of Molecular Oxygen on Polyphenol Oxidase-Modified Electrode
Creators:
Jiang, Hui
Shi, Chun-Hua
Xie, Yong-Shu
Liu, Qing-Liang
Uncontrolled Keywords:Polyphenol oxidase, Modified electrodes, Electrochemistry, Nafion modified electrode, Enzymes, Square-wave voltammetry, Oxygen detection
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:70
Number:3
Page Range:pp. 305-312
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc20050305UNSPECIFIED
ID Code:2360
Item Type:Article
Deposited On:06 Feb 2009 17:18
Last Modified:06 Feb 2009 16:18

Citation

Jiang, Hui; Shi, Chun-Hua; Xie, Yong-Shu; Liu, Qing-Liang (2005) Investigations on the Reduction of Molecular Oxygen on Polyphenol Oxidase-Modified Electrode. Collection of Czechoslovak Chemical Communications, 70 (3). pp. 305-312. ISSN 0010-0765

Repository Staff Only: item control page