NMR and X-ray Diffraction Study of the Conformational Structure of Poly[N5-(2-hydroxyethyl)-L-glutamine] and Poly[N5-(2-hydroxyethyl)-L-glutamine-co-γ-L-glutamylhydrazide]

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Title:NMR and X-ray Diffraction Study of the Conformational Structure of Poly[N5-(2-hydroxyethyl)-L-glutamine] and Poly[N5-(2-hydroxyethyl)-L-glutamine-co-γ-L-glutamylhydrazide]
Creators:
Hlavatá, Drahomíra
Pivcová, Hana
Spěváček, Jiří
Nosková, Dagmar
Rypáček, František
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 58, 10, pp. 2415-2427

Abstract

The conformation of poly[<i>N</i><sup>5</sup>-(2-hydroxyethyl)L-glutamine] (P(HEG-GH)), in solid state was studied by <sup>13</sup>C CP/MAS NMR spectrometry and X-ray diffraction. The association of copolymers in dichloroacetic acid was investigated by following the temperature dependence of their <sup>1</sup>H NMR spectra, PHEG and P(HEG-GH) were prepared by the reaction of poly(benzyl γ-L-glutamate) with 2-aminoethanol and 2-aminoethanol-hydrazine mixtures, respectively. The conformation of the copolymers depends on the reaction conditions. While PHEG and the copolymers prepared with the mole ratio of hydrazine to the benzyl ester <i>n</i><sub>H</sub>/<i>n</i><sub>B</sub> = 0.25 were almost amorphous in solid state and had a random coil conformation and/or randomly oriented helices, at increased concentration of hydrazine (<i>n</i><sub>H</sub>/<i>n</i><sub>B</sub> = 0.5 and 1.0) P(HEG-GH) formed more or less ordered structure characterized by assemblies of α-helices. The copolymers prepared at <i>n</i><sub>H</sub>/<i>n</i><sub>B</sub> 3 2.0 showed features of β-sheets. The tendency of the copolymers to form associates in Cl<sub>2</sub>CHCOOH solutions correlated well with their tendency to form ordered structures in solid state.

Title:NMR and X-ray Diffraction Study of the Conformational Structure of Poly[N5-(2-hydroxyethyl)-L-glutamine] and Poly[N5-(2-hydroxyethyl)-L-glutamine-co-γ-L-glutamylhydrazide]
Creators:
Hlavatá, Drahomíra
Pivcová, Hana
Spěváček, Jiří
Nosková, Dagmar
Rypáček, František
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:58
Number:10
Page Range:pp. 2415-2427
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19932415UNSPECIFIED
ID Code:358
Item Type:Article
Deposited On:06 Feb 2009 17:00
Last Modified:06 Feb 2009 16:00

Citation

Hlavatá, Drahomíra; Pivcová, Hana; Spěváček, Jiří; Nosková, Dagmar; Rypáček, František (1993) NMR and X-ray Diffraction Study of the Conformational Structure of Poly[N5-(2-hydroxyethyl)-L-glutamine] and Poly[N5-(2-hydroxyethyl)-L-glutamine-co-γ-L-glutamylhydrazide]. Collection of Czechoslovak Chemical Communications, 58 (10). pp. 2415-2427. ISSN 0010-0765

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