Purification of Penicillin Amidohydrolase, an Enzyme for Semisynthetic Procedures

[img]PDF - Authorized users only
Language: English
229Kb
Title:Purification of Penicillin Amidohydrolase, an Enzyme for Semisynthetic Procedures
Creators:
Jiráček, Jiří
Barth, Tomislav
Velek, Jiří
Bláha, Ivo
Pospíšek, Jan
Svoboda, Ivan
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 57, 10, pp. 2187-2191

Abstract

Penicillin amidohydrolase (EC 3.5.1.11.) is one of the few enzymes used successfully for deprotection of primary amino groups of semisynthetic peptides. The available material is usually contamined by endo- and exopeptidases. We managed to prepare the enzyme devoid of trypsin- and chymotrypsin-like activities using affinity chromatography with specific ligands: Gly-D-Phe-Phe-Tyr-Thr-Pro-Lys-Thr (the fF peptide) and Leu-Gly-Val-D-Arg-Arg-Gly-Phe (the rR peptide). For further purification of the enzyme affinity chromatography with N-phenylacetyl-D-tert-Leu as a ligand was used.

Title:Purification of Penicillin Amidohydrolase, an Enzyme for Semisynthetic Procedures
Creators:
Jiráček, Jiří
Barth, Tomislav
Velek, Jiří
Bláha, Ivo
Pospíšek, Jan
Svoboda, Ivan
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:57
Number:10
Page Range:pp. 2187-2191
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19922187UNSPECIFIED
ID Code:3881
Item Type:Article
Deposited On:06 Feb 2009 16:49
Last Modified:06 Feb 2009 15:54

Citation

Jiráček, Jiří; Barth, Tomislav; Velek, Jiří; Bláha, Ivo; Pospíšek, Jan; Svoboda, Ivan (1992) Purification of Penicillin Amidohydrolase, an Enzyme for Semisynthetic Procedures. Collection of Czechoslovak Chemical Communications, 57 (10). pp. 2187-2191. ISSN 0010-0765

Repository Staff Only: item control page