Nanoelectrospray versus electrospray in chiral analysis by the kinetic method

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Title:Nanoelectrospray versus electrospray in chiral analysis by the kinetic method
Creators:
Ranc, Václav
Havlíček, Vladimír
Bednář, Petr
Lemr, Karel
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 74, 2, pp. 313-322
Uncontrolled Keywords:Diastereoisomeric clusters, Drugs, Electrospray, Amino acids, Mass spectrometry, Nanoelectrospray, Chiral analysis, Kinetic method, Gas-phase

Abstract

Electrospray ionization generates trimeric diastereoisomeric clusters as the first important step in chiral analysis by mass spectrometry using the Cooks′ kinetic method. Cu<sup>2+</sup> and L-tryptophan were used as a central metal and as a chiral reference ligand, respectively. The comparison of electrospray and nanoelectrospray showed that although the electrospray system was generally more robust, the application of nanoelectrospray was essential for performing successful analysis in some cases, especially for real samples. Basically, no significant differences between the ion sources were observed for model samples of analytes (isoleucine, ephedrine, phenylalanine) without interfering matrix. On the other hand, model samples containing sodium chloride and a buffer containing a real sample (drug formulation Mucoseptonex E in which D-ephedrine is the active substance) could not be analyzed using ESI, whereas nano-ESI gave satisfactory results. An explanation is based on the differences of ionization processes occurring in the compared sources.

Title:Nanoelectrospray versus electrospray in chiral analysis by the kinetic method
Creators:
Ranc, Václav
Havlíček, Vladimír
Bednář, Petr
Lemr, Karel
Uncontrolled Keywords:Diastereoisomeric clusters, Drugs, Electrospray, Amino acids, Mass spectrometry, Nanoelectrospray, Chiral analysis, Kinetic method, Gas-phase
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:74
Number:2
Page Range:pp. 313-322
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc2008201UNSPECIFIED
ID Code:4100
Item Type:Article
Deposited On:02 Mar 2009 10:34
Last Modified:02 Mar 2009 09:34

Citation

Ranc, Václav; Havlíček, Vladimír; Bednář, Petr; Lemr, Karel (2009) Nanoelectrospray versus electrospray in chiral analysis by the kinetic method. Collection of Czechoslovak Chemical Communications, 74 (2). pp. 313-322. ISSN 0010-0765

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