Essential Arginine Residues in Lactate Dehydrogenase from Germinating Soybean

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Title:Essential Arginine Residues in Lactate Dehydrogenase from Germinating Soybean
Creators:
Barthová, Jana
Hulová, Irena
Birčáková, Miroslava
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 59, 2, pp. 467-472

Abstract

<p>The lactate dehydrogenase was isolated from soybean (<i>Glycine max.</i> L.) by a procedure that employed biospecific chromatography on a column of Blue-Sepharose CL-6B. The participation of the guanidine group of arginine residues in the mechanism of enzyme action was determined through kinetic and chemical modification studies. The dependence of enzyme activity on pH was followed in the alkaline region (pH 8.6 - 12.8). The p<i>K</i> values found were 12.4 for the enzyme substrate complex and 11.1 for the free enzyme. The enzyme was inactivated by phenylglyoxal, 2,3-butanedione, 1,2-cyclohexanedione and <i>p</i>-hydroxyphenylglyoxal reagents used in modification experiments. Kinetic analysis of the modification indicated that one arginine residue is modified when inactivation occurs. No effect was observed on the rate of inactivation upon addition of coenzyme. The extent of enzyme modification by <i>p</i>-hydroxyphenylglyoxal was determined. It appears there are at least two arginine residues in the active site of the enzyme.

Title:Essential Arginine Residues in Lactate Dehydrogenase from Germinating Soybean
Creators:
Barthová, Jana
Hulová, Irena
Birčáková, Miroslava
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:59
Number:2
Page Range:pp. 467-472
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19940467UNSPECIFIED
ID Code:465
Item Type:Article
Deposited On:06 Feb 2009 17:01
Last Modified:06 Feb 2009 16:01

Citation

Barthová, Jana; Hulová, Irena; Birčáková, Miroslava (1994) Essential Arginine Residues in Lactate Dehydrogenase from Germinating Soybean. Collection of Czechoslovak Chemical Communications, 59 (2). pp. 467-472. ISSN 0010-0765

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