Voltammetric and amperometric determination of doxorubicin using carbon paste electrodes

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Title:Voltammetric and amperometric determination of doxorubicin using carbon paste electrodes
Creators:
Jemelková, Zuzana
Zima, Jiří
Barek, Jiří
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 74, 10, pp. 1503-1515
Uncontrolled Keywords:Carbon paste electrode, Direct current voltammetry, Differential pulse voltammetry, Doxorubicin, HPLC with amperometric detection

Abstract

Direct current voltammetric (DCV) and differential pulse voltammetric (DPV) determination of antineoplastic agent doxorubicin (DOX) at a carbon paste electrode (CPE) was developed. Britton–Robinson buffer (pH 7.0) was used as a supporting electrolyte. The limits of detection are 8 × 10<sup>–7</sup> mol l<sup>–1</sup> (DCV) and 6 × 10<sup>–8</sup> mol l<sup>–1</sup> (DPV). The accumulation of DOX at the electrode surface was used to decrease the limits of detection down to 2.2 × 10<sup>–7</sup> mol l<sup>–1</sup> for adsorptive stripping DC voltammetry (DCAdSV) and 2.8 × 10<sup>–9</sup> mol l<sup>–1</sup> for adsorptive stripping differential pulse voltammetry (DPAdSV) at CPE. The results of the voltammetric methods were utilized for the development of a new determination of doxorubicin using HPLC with amperometric detection on CPE based on spherical microparticles of glassy carbon in a wall-jet configuration. A column with chemically bonded C18 stationary phase and a mobile phase containing 0.01 M phosphate buffer (pH 5.0)–methanol 25:75 (v/v) were used. The limit of detection is 4 × 10<sup>–7</sup> mol l<sup>–1</sup> (HPLC with electrochemical detection (ED)).

Title:Voltammetric and amperometric determination of doxorubicin using carbon paste electrodes
Creators:
Jemelková, Zuzana
Zima, Jiří
Barek, Jiří
Uncontrolled Keywords:Carbon paste electrode, Direct current voltammetry, Differential pulse voltammetry, Doxorubicin, HPLC with amperometric detection
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:74
Number:10
Page Range:pp. 1503-1515
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc2009081UNSPECIFIED
ID Code:4782
Item Type:Article
Deposited On:17 Dec 2009 12:41
Last Modified:17 Dec 2009 11:41

Citation

Jemelková, Zuzana; Zima, Jiří; Barek, Jiří (2009) Voltammetric and amperometric determination of doxorubicin using carbon paste electrodes. Collection of Czechoslovak Chemical Communications, 74 (10). pp. 1503-1515. ISSN 0010-0765

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