The reduction of doxorubicin at a mercury electrode and monitoring its interaction with DNA using constant current chronopotentiometry

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Title:The reduction of doxorubicin at a mercury electrode and monitoring its interaction with DNA using constant current chronopotentiometry
Creators:
Vacek, Jan
Havran, Luděk
Fojta, Miroslav
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 74, 11-12, pp. 1727-1738
Uncontrolled Keywords:Chronopotentiometry, Anthracyclins, Mercury electrode, Adsorption, Antibiotics, Plasmid DNA, Reduction, Doxorubicin, Voltammetry, Electrochemistry

Abstract

In this report, voltammetry with linear scan and chronopotentiometric stripping (CPS) with constant current were used for the analysis of doxorubicin (DOX) at a hanging mercury drop electrode (HMDE). CPS was used for the study of DOX <i>in situ</i> electrochemical reduction in adsorbed state and for <i>ex situ</i> (adsorptive transfer) analysis of the drug. For the first time, CPS was used to study the reversible reduction of the DOX quinine moiety at –0.45 V (vs Ag|AgCl|3 M KCl) as well as electrode processes giving rise to an irreversible signal around –1.45 V at the HMDE in 0.2 M acetate or Britton–Robinson buffers at different pH values. The dependence of the latter signal on pH revealed involvement of protonation equilibria; however, neither CV nor CPS data confirmed the catalytic character of the electrode reaction previously suggested by other authors. The CPS method was also applied to monitor the DOX interaction with double- (ds) and single-stranded (ss) DNA. In the presence of dsDNA, more pronounced changes in DOX signal intensity were observed, in agreement with a strong intercalation of the DOX redox centre into the DNA double helix.

Title:The reduction of doxorubicin at a mercury electrode and monitoring its interaction with DNA using constant current chronopotentiometry
Creators:
Vacek, Jan
Havran, Luděk
Fojta, Miroslav
Uncontrolled Keywords:Chronopotentiometry, Anthracyclins, Mercury electrode, Adsorption, Antibiotics, Plasmid DNA, Reduction, Doxorubicin, Voltammetry, Electrochemistry
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:74
Number:11-12
Page Range:pp. 1727-1738
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc2009512UNSPECIFIED
ID Code:4859
Item Type:Article
Deposited On:17 Feb 2010 16:45
Last Modified:17 Feb 2010 15:45

Citation

Vacek, Jan; Havran, Luděk; Fojta, Miroslav (2009) The reduction of doxorubicin at a mercury electrode and monitoring its interaction with DNA using constant current chronopotentiometry. Collection of Czechoslovak Chemical Communications, 74 (11-12). pp. 1727-1738. ISSN 0010-0765

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