From DC polarographic presodium wave of proteins to electrochemistry of biomacromolecules

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Title:From DC polarographic presodium wave of proteins to electrochemistry of biomacromolecules
Creators:
Paleček, Emil
Heyrovský, Michael
Janík, Bořivoj
Kaláb, Dušan
Pechan, Zdeněk
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 74, 11-12, pp. 1739-1755
Uncontrolled Keywords:Chronopotentiometry, Proteins, Polarography, Presodium wave, Nucleic acids, Electrochemistry

Abstract

History of electrochemistry of proteins and nucleic acids is briefly reviewed. The ability of proteins to catalyze hydrogen evolution at Hg electrodes was discovered almost 80 years ago in J. Heyrovský’s laboratory. This phenomenon was not sufficiently appreciated for several decades. Recently it has been shown that using constant current chronopotentiometric stripping (CPS) with hanging mercury drop, solid amalgam or Hg-film electrodes the CPS peak H is obtained with nanomolar concentrations of peptides and proteins. This peak is derived from the presodium wave but it has some new properties useful in protein research. It is sensitive to changes in protein structures and to protein redox states, representing a new tool for protein analysis applicable in biomedicine. Electroactivity of nucleic acids was discovered about 50 years ago. Electrochemistry of DNA and RNA is now a booming field because of its potential use in sensors for DNA hybridization and DNA damage. Quite recently it has been shown that electrochemistry can be applied also in polysaccharide analysis. A review with 99 references.

Title:From DC polarographic presodium wave of proteins to electrochemistry of biomacromolecules
Creators:
Paleček, Emil
Heyrovský, Michael
Janík, Bořivoj
Kaláb, Dušan
Pechan, Zdeněk
Uncontrolled Keywords:Chronopotentiometry, Proteins, Polarography, Presodium wave, Nucleic acids, Electrochemistry
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:74
Number:11-12
Page Range:pp. 1739-1755
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc2009511UNSPECIFIED
ID Code:4860
Item Type:Article
Deposited On:17 Feb 2010 16:45
Last Modified:17 Feb 2010 15:45

Citation

Paleček, Emil; Heyrovský, Michael; Janík, Bořivoj; Kaláb, Dušan; Pechan, Zdeněk (2009) From DC polarographic presodium wave of proteins to electrochemistry of biomacromolecules. Collection of Czechoslovak Chemical Communications, 74 (11-12). pp. 1739-1755. ISSN 0010-0765

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