Electrochemical and Spectrophotometric Study of the Reactions of L-Leucine with Pyridoxal and Pyridoxal Phosphate

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Title:Electrochemical and Spectrophotometric Study of the Reactions of L-Leucine with Pyridoxal and Pyridoxal Phosphate
Creators:
Pineda, T.
Blázquez, M.
Domínguez, M.
Garcia-Blanco, F.
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 59, 4, pp. 768-781

Abstract

<p>The reactions of L-leucine (Leu) with pyridoxal (PL) and pyridoxal-5'-phosphate (PLP) were studied over pH 6 - 13.5. Formation of the Schiff bases was indicated by UV-VIS absorption bands and reduction waves on a mercury electrode. A comparative electrochemical and spectrophotometric study on the reaction mixtures as a function of pH and the amino acid-to-aldehyde concentration ratio was carried out. Apparent formation constant, <i>K</i><sub>pH</sub> and acid-base constants for the Schiff bases were obtained. Reduction mechanisms of the imines are proposed in different pH zones. The formation constant, <i>K</i><sub>So</sub>, of an unprotonated Schiff base from unprotonated species of aldehyde and amino acid was determined by voltammetry. The results are compared to those obtained in analog Schiff bases in order to improve the quantitative description of the enzymatic models.

Title:Electrochemical and Spectrophotometric Study of the Reactions of L-Leucine with Pyridoxal and Pyridoxal Phosphate
Creators:
Pineda, T.
Blázquez, M.
Domínguez, M.
Garcia-Blanco, F.
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:59
Number:4
Page Range:pp. 768-781
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19940768UNSPECIFIED
ID Code:500
Item Type:Article
Deposited On:06 Feb 2009 17:01
Last Modified:06 Feb 2009 16:01

Citation

Pineda, T.; Blázquez, M.; Domínguez, M.; Garcia-Blanco, F. (1994) Electrochemical and Spectrophotometric Study of the Reactions of L-Leucine with Pyridoxal and Pyridoxal Phosphate. Collection of Czechoslovak Chemical Communications, 59 (4). pp. 768-781. ISSN 0010-0765

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