Hydrophobic partitioning of proteins in a two-phase aqueous system of poly(oxyethylene)-dextran alternatively derivatized by 2-hydroxy-3-phenoxypropyl group

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Title:Hydrophobic partitioning of proteins in a two-phase aqueous system of poly(oxyethylene)-dextran alternatively derivatized by 2-hydroxy-3-phenoxypropyl group
Creators:
Gemeiner, Peter
Kochjarová, Helena
Horváthová, Marta
Breier, Albert
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 56, 6, pp. 1270-1278

Abstract

We have observed that in a two-phase aqueous system composed of poly(oxyethylene) and dextran, trypsin, ovalbumin, serum albumin and immunoglobulin G are preferentially partitioned into the dextran phase. The accumulation of proteins in this more hydrophilic phase increased with the increasing concentration of the polymers in both phases. This trend remained essentially unchanged even after the hydrophobization of the poly(oxyethylene) (POE) phase by 2-hydroxy-3-phenoxypropyl (HPP). The affinity of all four proteins for the dextran phase decreased after the hydrophobization of the dextran phase by the HPP ligand. The affinity decrease was uniform with all four proteins. The hydrophobic partitioning of all four proteins in the systems composed of (HPP-POE) and dextran and of POE and (HPP-dextran) was probably governed by an identical mechanism. The size and charge of the globular protein played an important role in this mechanism.

Title:Hydrophobic partitioning of proteins in a two-phase aqueous system of poly(oxyethylene)-dextran alternatively derivatized by 2-hydroxy-3-phenoxypropyl group
Creators:
Gemeiner, Peter
Kochjarová, Helena
Horváthová, Marta
Breier, Albert
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:56
Number:6
Page Range:pp. 1270-1278
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19911270UNSPECIFIED
ID Code:5048
Item Type:Article
Deposited On:22 Feb 2010 11:29
Last Modified:22 Feb 2010 10:29

Citation

Gemeiner, Peter; Kochjarová, Helena; Horváthová, Marta; Breier, Albert (1991) Hydrophobic partitioning of proteins in a two-phase aqueous system of poly(oxyethylene)-dextran alternatively derivatized by 2-hydroxy-3-phenoxypropyl group. Collection of Czechoslovak Chemical Communications, 56 (6). pp. 1270-1278. ISSN 0010-0765

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