Mushroom tyrosinase-modified carbon paste electrode as an amperometric biosensor for phenols

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Title:Mushroom tyrosinase-modified carbon paste electrode as an amperometric biosensor for phenols
Creators:
Skládal, Petr
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 56, 7, pp. 1427-1433

Abstract

A biosensor for a variety of substances could be readily prepared by the adsorption of partially purified mushroom tyrosinase at a carbon paste electrode, producing an enzyme layer which was protected with a dialysis membrane. The electrode poised at – 100mV (vs an Ag/AgCl reference electrode) functions on the basis of a reversible electrochemical reduction of the <i>o</i>-quinones formed from phenols in the tyrosinase reaction. The response times were 40 s for <i>o</i>-diphenols and 2 min for monophenols; the reproducibility for 10 consecutive assays of 3 μmol l<sup>-1</sup> phenol solution was better than 4%. The detection limit was 10 nmol l<sup>-1</sup> (catechol as substrate) and the steady state responses were linear up to a concentration of 200 μmol l<sup>-1</sup>. The optimum pH values were 6.0 for phenol and 6.5 for catechol. The shapes of the pH-response curves indicated the presence of at least two tyrosinase isoenzymes with different pH optima.

Title:Mushroom tyrosinase-modified carbon paste electrode as an amperometric biosensor for phenols
Creators:
Skládal, Petr
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:56
Number:7
Page Range:pp. 1427-1433
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19911427UNSPECIFIED
ID Code:5064
Item Type:Article
Deposited On:22 Feb 2010 11:29
Last Modified:22 Feb 2010 10:30

Citation

Skládal, Petr (1991) Mushroom tyrosinase-modified carbon paste electrode as an amperometric biosensor for phenols. Collection of Czechoslovak Chemical Communications, 56 (7). pp. 1427-1433. ISSN 0010-0765

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