Use of inhibited enzyme electrode for estimation of PEA diamine oxidase inhibitors

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Title:Use of inhibited enzyme electrode for estimation of PEA diamine oxidase inhibitors
Creators:
Macholán, Lumír
Slanina, Jiří
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 56, 7, pp. 1545-1551

Abstract

A biosensor consisting of a Clark type oxygen electrode and immobilized pea diamine oxidase makes it possible amperometrically to quantify, on the basis of an inhibition effect, substrate analogues of the enzyme (aliphatic amino ketones and monoamines, aromatic diamines), some alkaloids (cinchonine, lobeline, norsedamine) and drugs (1-phenylcyclopropylamine, naphazoline). The output current signal of the inhibited bioelectrode is influenced by the thickness of the membrane and its enzyme content as well as by the sort of the substrate and oxygen concentration in the reaction medium. With most of the tested substances, the response time τ<sub>90</sub> is 20-100 s and the linear concentration range about 10<sup>-6</sup>-10<sup>-3</sup> mol l<sup>-1</sup> can be observed. The inhibitor concentrations causing a change of the initial current by 50% correspond mostly to the published values of inhibition constants for soluble enzyme. The most efficient inhibitor of the pea diamine oxidase is 1,5-diamino-3-pentanone, for which the accuracy of the method was 5.6%, its reproducibility 7.2%, and detection limit 5 . 10<sup>-9</sup> mol l<sup>-1</sup>.

Title:Use of inhibited enzyme electrode for estimation of PEA diamine oxidase inhibitors
Creators:
Macholán, Lumír
Slanina, Jiří
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:56
Number:7
Page Range:pp. 1545-1551
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19911545UNSPECIFIED
ID Code:5078
Item Type:Article
Deposited On:22 Feb 2010 11:29
Last Modified:22 Feb 2010 10:30

Citation

Macholán, Lumír; Slanina, Jiří (1991) Use of inhibited enzyme electrode for estimation of PEA diamine oxidase inhibitors. Collection of Czechoslovak Chemical Communications, 56 (7). pp. 1545-1551. ISSN 0010-0765

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