Synthetic Peptides from the Region 30-54 of the Interleukin-2 Receptor β-Chain (IL-2Rβ)Affect IL-2 Binding to Human T-Cells

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Title:Synthetic Peptides from the Region 30-54 of the Interleukin-2 Receptor β-Chain (IL-2Rβ)Affect IL-2 Binding to Human T-Cells
Creators:
Drakopoulou, Eugenia
Sotiropoulou, Georgia
Tsilivakos, Vassilis
Georgoulias, Vassilis
Cordopatis, Paul
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 59, 4, pp. 943-950

Abstract

<p>The interleukin-2 receptor β-chain (IL-2Rβ) is responsible for the IL-2 internalization and signal transduction. To identify domains from the IL-2Rβ extracellular region which are involved in ligand receptor interactions, eight peptides of 8 to 25 amino acids corresponding to IL-2Rβ sequence 30-54 were synthesized. Their biological effect was tested with [<sup>125</sup>I]IL-2 equilibrium binding to PHA-blasts. Preincubation of [<sup>125</sup>I]IL-2 with peptides IL-2Rβ (35-54) and (30-42) affects IL-2 binding to both high and intermediate affinity receptors by revealing a greater number of active sites with lower affinity.

Title:Synthetic Peptides from the Region 30-54 of the Interleukin-2 Receptor β-Chain (IL-2Rβ)Affect IL-2 Binding to Human T-Cells
Creators:
Drakopoulou, Eugenia
Sotiropoulou, Georgia
Tsilivakos, Vassilis
Georgoulias, Vassilis
Cordopatis, Paul
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:59
Number:4
Page Range:pp. 943-950
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19940943UNSPECIFIED
ID Code:513
Item Type:Article
Deposited On:06 Feb 2009 17:01
Last Modified:06 Feb 2009 16:01

Citation

Drakopoulou, Eugenia; Sotiropoulou, Georgia; Tsilivakos, Vassilis; Georgoulias, Vassilis; Cordopatis, Paul (1994) Synthetic Peptides from the Region 30-54 of the Interleukin-2 Receptor β-Chain (IL-2Rβ)Affect IL-2 Binding to Human T-Cells. Collection of Czechoslovak Chemical Communications, 59 (4). pp. 943-950. ISSN 0010-0765

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