Circularly polarized luminiscence as a probe of metal ions binding sites in calmodulin

[img]PDF - Authorized users only
Language: English
140Kb
Title:Circularly polarized luminiscence as a probe of metal ions binding sites in calmodulin
Creators:
Coruh, Nursen
Riehl, James P.
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 56, 12, pp. 3028-3031

Abstract

Circularly polarized luminiscence (CPL) from dilute solutions of Tb(III) bound to the Ca-binding protein calmodulin is reported. The dissymmetry ratio, <i>g</i><sub>em</sub>, at 543.5 nm can be monitored as a function of equivalents of metal ion concentration. Competitive and consecutive addition of Ca(II) versus Tb(III) yield results which are consistent with previous results that suggest that Tb(III) and Ca(II) have a preferred affinity for different metal-ion binding sites in this protein.

Title:Circularly polarized luminiscence as a probe of metal ions binding sites in calmodulin
Creators:
Coruh, Nursen
Riehl, James P.
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:56
Number:12
Page Range:pp. 3028-3031
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19913028UNSPECIFIED
ID Code:5210
Item Type:Article
Deposited On:22 Feb 2010 11:32
Last Modified:22 Feb 2010 10:33

Citation

Coruh, Nursen; Riehl, James P. (1991) Circularly polarized luminiscence as a probe of metal ions binding sites in calmodulin. Collection of Czechoslovak Chemical Communications, 56 (12). pp. 3028-3031. ISSN 0010-0765

Repository Staff Only: item control page