Polarographic determination of vitamin C after derivatization with o-phenylenediamine

[img]PDF - Authorized users only
Language: English
1008Kb
Title:Polarographic determination of vitamin C after derivatization with o-phenylenediamine
Creators:
Rodrigues, José António
Valente, Inês Maria
Gonçalves, Luís Moreira
Pacheco, João Grosso
Barros, Aquiles Araújo
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 75, 7, pp. 731-741
Uncontrolled Keywords:Fruit juices, Vitamin C, Polarography, Ascorbic acid, Voltammetry, Electrochemical techniques

Abstract

A differential pulse polarographic (DPP) method has been developed for the determination of ascorbic acid (AA) and dehydroascorbic acid (DHA), the two main forms of Vitamin C. The method consists of the DPP analysis of a quinoxaline obtained by the derivatization of DHA with <i>o</i>-phenylenediamine. Results using the proposed method correlated well with those obtained by two reference methodologies: the common iodometric method and a published chromatographic methodology. It was also used in the study of Vitamin C degradation in fruit juices, showing that it involves an initial oxidation of AA to DHA, followed by hydrolytic degradation of the latter.

Title:Polarographic determination of vitamin C after derivatization with o-phenylenediamine
Creators:
Rodrigues, José António
Valente, Inês Maria
Gonçalves, Luís Moreira
Pacheco, João Grosso
Barros, Aquiles Araújo
Uncontrolled Keywords:Fruit juices, Vitamin C, Polarography, Ascorbic acid, Voltammetry, Electrochemical techniques
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:75
Number:7
Page Range:pp. 731-741
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc2010026UNSPECIFIED
ID Code:6187
Item Type:Article
Deposited On:11 Oct 2010 15:42
Last Modified:11 Oct 2010 13:42

Citation

Rodrigues, José António; Valente, Inês Maria; Gonçalves, Luís Moreira; Pacheco, João Grosso; Barros, Aquiles Araújo (2010) Polarographic determination of vitamin C after derivatization with o-phenylenediamine. Collection of Czechoslovak Chemical Communications, 75 (7). pp. 731-741.

Repository Staff Only: item control page