Electroanalysis of some catecholamines at a single-wall nanotubes modified carbon paste electrode

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Title:Electroanalysis of some catecholamines at a single-wall nanotubes modified carbon paste electrode
Creators:
Jemelková, Zuzana
Zima, Jiří
Barek, Jiří
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 75, 12, pp. 1217-1228
Uncontrolled Keywords:HPLC-ED, Carbon paste electrode, Catecholamines, Differential pulse voltammetry, Voltammetry, Electrochemistry, HPLC, Carbon nanotubes

Abstract

Catecholamines (CAs) are important neurotransmitters and hormones in mammalian species. They are highly reactive and are readily oxidized to aminochromes. That enables the use of electrochemical method for their determination. In this paper, differential pulse voltammetric (DPV) determination and HPLC with amperometric detection of dopamine (D), epinephrine (E), norepinephrine (NE) and <span class="small_cap">L</span>-Dopa (DOPA) is developed and optimized. The carbon paste electrode (CPE) prepared of carbon powder CR2 and single-wall carbon nanotubes (SWCNT) (50:50 m/m) was used. The miniaturized body for CPE was used to reduce consumption of all chemicals. In Britton–Robinson buffer solution pH 6, the limits of detection 1 × 10<sup>–6</sup>, 2 × 10<sup>–6</sup>, 2 × 10<sup>–6</sup> and 8 × 10<sup>–6</sup> mol dm<sup>–3</sup> have been found for DPV determination of D, E, NE and DOPA, respectively. HPLC was carried out using 0.1 <span class="small_cap">M</span> phosphate buffer pH 3:methanol (95:5) mobile phase containing 10 m<span class="small_cap">M</span> EDTA. The above described CPE in a wall-jet arrangement was used for amperometric detection. The limit of detection of each analyte in a mixture was 2 × 10<sup>–6</sup> mol dm<sup>–3</sup>. Practical application of this method on spiked urine samples was verified.

Title:Electroanalysis of some catecholamines at a single-wall nanotubes modified carbon paste electrode
Creators:
Jemelková, Zuzana
Zima, Jiří
Barek, Jiří
Uncontrolled Keywords:HPLC-ED, Carbon paste electrode, Catecholamines, Differential pulse voltammetry, Voltammetry, Electrochemistry, HPLC, Carbon nanotubes
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:75
Number:12
Page Range:pp. 1217-1228
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc2010076UNSPECIFIED
ID Code:6326
Item Type:Article
Deposited On:01 Dec 2010 18:42
Last Modified:01 Dec 2010 17:42

Citation

Jemelková, Zuzana; Zima, Jiří; Barek, Jiří (2010) Electroanalysis of some catecholamines at a single-wall nanotubes modified carbon paste electrode. Collection of Czechoslovak Chemical Communications, 75 (12). pp. 1217-1228.

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