Thermal Stability of Human Blood Serum Orosomucoid (Acid α1-Glycoprotein) in Water and Water-Ethanol Systems

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Title:Thermal Stability of Human Blood Serum Orosomucoid (Acid α1-Glycoprotein) in Water and Water-Ethanol Systems
Creators:
Janáčková, Ludmila
Karpenko, Vladimír
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 59, 10, pp. 2190-2200

Abstract

<p>Thermal stability of human serum orosomucoid (acid α<sub>1</sub>-glycoprotein) was studied in water and in water - ethanol systems by the differential scanning microcalorimetry and temperature perturbation difference spectrometry. This investigation has led to the following results: (i) the change in heat capacity <i>C</i><sub>p</sub> of isoionic orosomucoid with temperature was irreversible; different values of ∆<i>H</i> were obtained on repeated heating; (ii) in buffered solution at pH 7, hysteresis in temperature perturbation difference spectra was observed within the wavelength range of 250 - 266 nm; (iii) a strong destabilizing effect of ethanol on the orosomucoid molecule was observed in the alkaline region at an alcohol volume fraction of 20%. This effect corresponds to the anomalous behaviour of the water ethanol systems.

Title:Thermal Stability of Human Blood Serum Orosomucoid (Acid α1-Glycoprotein) in Water and Water-Ethanol Systems
Creators:
Janáčková, Ludmila
Karpenko, Vladimír
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:59
Number:10
Page Range:pp. 2190-2200
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19942190UNSPECIFIED
ID Code:641
Item Type:Article
Deposited On:06 Feb 2009 17:02
Last Modified:06 Feb 2009 16:02

Citation

Janáčková, Ludmila; Karpenko, Vladimír (1994) Thermal Stability of Human Blood Serum Orosomucoid (Acid α1-Glycoprotein) in Water and Water-Ethanol Systems. Collection of Czechoslovak Chemical Communications, 59 (10). pp. 2190-2200. ISSN 0010-0765

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