Enzymatically Catalyzed Generation of Electronically Excited States in a Synthetic Copolymer with a Schiff Base in Pendant Groups

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Title:Enzymatically Catalyzed Generation of Electronically Excited States in a Synthetic Copolymer with a Schiff Base in Pendant Groups
Creators:
Zoulík, Robert
Pavlíček, Zdeněk
Vohlídal, Jiří
Šubr, Vladimír
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 60, 1, pp. 95-103

Abstract

<p>The copolymer of <i>N</i>-(2-hydroxypropyl)methacrylamide and <i>N</i>-[<i>N</i>-(<i>N</i>-methacryloylglycyl)glycyl]hexane- 1,6-diamine (17.6 mole %) was prepared as a model compound for investigation of the mechanism of enzymatically catalyzed generation of electronically excited states in proteins. The amino end groups of the copolymer side chains were transformed into a Schiff base by reaction with glycolaldehyde. The modified copolymer was characterized by its ultraviolet, visible and fluorescence spectra. The enzymatically catalyzed oxidation of the modified copolymer was carried out in a phosphate buffer at pH 7 using horseradish peroxidase as the enzyme. The generation of electronically excited states was demonstrated by chemiluminescence measurement and by a spectroscopic procedure based on transfer of the excitation energy to bilirubin.

Title:Enzymatically Catalyzed Generation of Electronically Excited States in a Synthetic Copolymer with a Schiff Base in Pendant Groups
Creators:
Zoulík, Robert
Pavlíček, Zdeněk
Vohlídal, Jiří
Šubr, Vladimír
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:60
Number:1
Page Range:pp. 95-103
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19950095UNSPECIFIED
ID Code:701
Item Type:Article
Deposited On:06 Feb 2009 17:03
Last Modified:06 Feb 2009 16:03

Citation

Zoulík, Robert; Pavlíček, Zdeněk; Vohlídal, Jiří; Šubr, Vladimír (1995) Enzymatically Catalyzed Generation of Electronically Excited States in a Synthetic Copolymer with a Schiff Base in Pendant Groups. Collection of Czechoslovak Chemical Communications, 60 (1). pp. 95-103. ISSN 0010-0765

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