Synthesis of Fragments of the Vasoactive Intestinal Peptide (VIP) and Analysis of Their Residual Biological Activities

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Title:Synthesis of Fragments of the Vasoactive Intestinal Peptide (VIP) and Analysis of Their Residual Biological Activities
Creators:
Zoulíková, Ivana
Svoboda, Ivan
Velek, Jiří
Kašička, Václav
Slaninová, Jiřina
Barth, Tomislav
Hrbas, Pavel
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 60, 7, pp. 1229-1235

Abstract

<p>The vasoactive intestinal (poly)peptide (VIP) is a linear peptide containing 28 amino acid residues, whose primary structure indicates a low metabolic stability. The following VIP fragments, as potential metabolites, and their analogues were prepared by synthesis on a solid: [His(Dnp)<sup>1</sup>]VIP(1-10), VIP(11-14), [D-Arg<sup>12</sup>]VIP(11-14), [Lys(Pac)<sup>15,21</sup>,Arg<sup>20</sup>]VIP(15-22), and VIP(23-28). After purification, the peptides were characterized by amino acid analysis, mass spectrometry, RP HPLC, and capillary zone electrophoresis. In some tests, detailed examination of the biological activity of the substances in vivo and in vitro gave evidence of a low, residual activity of some fragments, viz. a depressoric activity in vivo for [His(Dnp)<sup>1</sup>]VIP(1-10) and a stimulating activity for the release of α-amylase in vitro and in vivo for [Lys(Pac)<sup>15,21</sup>,Arg<sup>20</sup>]VIP(15-22) and VIP(23-28).

Title:Synthesis of Fragments of the Vasoactive Intestinal Peptide (VIP) and Analysis of Their Residual Biological Activities
Creators:
Zoulíková, Ivana
Svoboda, Ivan
Velek, Jiří
Kašička, Václav
Slaninová, Jiřina
Barth, Tomislav
Hrbas, Pavel
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:60
Number:7
Page Range:pp. 1229-1235
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19951229UNSPECIFIED
ID Code:819
Item Type:Article
Deposited On:06 Feb 2009 17:04
Last Modified:06 Feb 2009 16:04

Citation

Zoulíková, Ivana; Svoboda, Ivan; Velek, Jiří; Kašička, Václav; Slaninová, Jiřina; Barth, Tomislav; Hrbas, Pavel (1995) Synthesis of Fragments of the Vasoactive Intestinal Peptide (VIP) and Analysis of Their Residual Biological Activities. Collection of Czechoslovak Chemical Communications, 60 (7). pp. 1229-1235. ISSN 0010-0765

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