Lipase Immobilized by Sol-Gel Technique in Layers

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Title:Lipase Immobilized by Sol-Gel Technique in Layers
Creators:
Kuncová, Gabriela
Guglielmi, Massimo
Dubina, Pavel
Šafář, Bohuslav
Journal or Publication Title:
Collection of Czechoslovak Chemical Communications, 60, 9, pp. 1573-1577

Abstract

<p>Commercial lipase was immobilized into an organic-inorganic matrix formed by hydrolysis of silicon alkoxides (tetraethoxysilane, dimethyldiethoxysilane and methyltriethoxysilane) with (3-aminopropyl)triethoxysilane, (3-thiopropyl)trimethoxysilane and chlorodiisopropyloctylsilane. Hydrolytic activity of lipase was tested after addition of the enzyme to a prepolymer solution, after gelation, in xerogel particles and in thin layers deposited on glass slides by dip- or spin-coating. The prepolymer containing NH groups showed the higher activity then the native enzyme.

Title:Lipase Immobilized by Sol-Gel Technique in Layers
Creators:
Kuncová, Gabriela
Guglielmi, Massimo
Dubina, Pavel
Šafář, Bohuslav
Divisions:Life and Chemical Sciences > Institute of Organic Chemistry and Biochemistry > Collection of Czechoslovak Chemical Communications
Journal or Publication Title:Collection of Czechoslovak Chemical Communications
Volume:60
Number:9
Page Range:pp. 1573-1577
ISSN:0010-0765
E-ISSN:1212-6950
Publisher:Institute of Organic Chemistry and Biochemistry
Related URLs:
URLURL Type
http://dx.doi.org/10.1135/cccc19951573UNSPECIFIED
ID Code:853
Item Type:Article
Deposited On:06 Feb 2009 17:04
Last Modified:06 Feb 2009 16:04

Citation

Kuncová, Gabriela; Guglielmi, Massimo; Dubina, Pavel; Šafář, Bohuslav (1995) Lipase Immobilized by Sol-Gel Technique in Layers. Collection of Czechoslovak Chemical Communications, 60 (9). pp. 1573-1577. ISSN 0010-0765

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